Open AccessModel for lactose repressor protein and its interaction with ligands.
Author(s) -
Marietta Dunaway,
Susan P. Manly,
Kathleen S. Matthews
Publication year - 1980
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.12.7181
Subject(s) - lac repressor , inducer , repressor , operator (biology) , dna , core protein , lac operon , chemistry , core (optical fiber) , binding site , biochemistry , computational biology , biology , genetics , biophysics , physics , gene , gene expression , optics , plasmid
A model is presented for the structure of the lactose repressor protein and for its interaction with inducer, operator DNA, and nonspecific DNA. The proposed structure is based on experimental evidence from this laboratory and from the literature and is offered as an integration of the available data on this system. Features unique to this model include: (i) interaction of the core region of the protein with the operator, (ii) primary effects of the conformational change in response to inducer on the core-operator interaction, (iii) contacts between all four subunits of the protein and the operator DNA, and (iv) qualitative differences in operator and nonspecific DNA binding.
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