Open AccessThe role of nuclear factor-κB essential modulator (NEMO) in B cell development and survival
Author(s) -
Shinyop Kim,
Ross N. A. La Motte-Mohs,
Dorothea Rudolph,
Juan Carlos ZúñigaPflücker,
Tak W. Mak
Publication year - 2003
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.0337707100
Subject(s) - transcription factor , microbiology and biotechnology , lymphopoiesis , biology , stem cell , b cell , nfkb1 , nf κb , iκb kinase , embryonic stem cell , in vitro , immunology , signal transduction , cancer research , haematopoiesis , genetics , antibody , gene
The transcription factor nuclear factor-kappaB (NF-kappaB) is essential for immune and inflammatory responses. NF-kappaB essential modulator (NEMO) is a scaffolding component of the IkappaB kinase complex required for NF-kappaB activation in vitro. Because NF-kappaB activation is involved in B cell development and function, we set out to determine whether NEMO is required for these processes. NEMO(-/-) mice die very early during embryogenesis, and fetal livers from NEMO(-/-) embryos can not reconstitute either B or T lymphopoiesis in irradiated host mice. We therefore used NEMO(-/-) embryonic stem cells and the OP9 in vitro differentiation system to demonstrate that NEMO is not required for B cell development but plays an important role in B cell survival.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom