Premium
Human neutrophil antigen‐2a expression on neutrophils from healthy adults in western Japan
Author(s) -
Taniguchi Kikuyo,
Kobayashi Masao,
Harada Hironori,
Hiraoka Asako,
Tanihiro Misae,
Takata Noboru,
Kimura Akiro
Publication year - 2002
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.2002.00092.x
Subject(s) - immunology , flow cytometry , antigen , population , antibody , phenotype , biology , epitope , medicine , andrology , biochemistry , environmental health , gene
BACKGROUND : Human neutrophil antigen‐2a (HNA‐2a)‐specific MoAb, which is necessary to prepare panel neutrophils and to determine the phenotype of patients' neutrophils, has been produced in many laboratories in Japan. The frequency of the HNA‐2a‐positive population in western Japan is unknown. STUDY DESIGN AND METHODS : A cell line secreting an HNA‐2a‐specific MoAb, TAG4, was established. TAG4 was characterized by flow cytometry, and the reactivities of TAG4 and another HNA‐2a‐specific MoAb, 7D8, were compared in an inhibition assay and two‐color flow cytometry. TAG4 was tested against granulocytes from 408 healthy adult volunteers in western Japan. RESULTS : TAG4 reacted with neutrophils, but not with the other blood cells. The reactivity of TAG4 with granulocytes treated with glycosyl‐phosphatidylinositol‐specific phospholipase C was markedly reduced. The reactivity of TAG4 and 7D8 was similar, but 7D8 did not prevent TAG4 from binding. A total of 406 of 408 donors were TAG4 positive. The mean percentage of HNA‐2a expression of females was 70.2 percent, and that of males was 66.8 percent. CONCLUSION : TAG4 and 7D8 seem to bind to different epitopes of HNA‐2a. The frequency of the HNA‐2a‐positive population is 99.5 percent of healthy adults in western Japan. The mean percentage of HNA‐2a expression of females was greater than in males.