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A rapid molecular diagnosis of posttransfusion graft‐versus‐host disease by polymerase chain reaction
Author(s) -
Hayakawa S.,
Chishima F.,
Sakata H.,
Fujii K.,
Ohtani K.,
Kurashina K.,
Hayakawa J.,
Suzuki K.,
Nakabayas H.,
Esumi M.,
Nemoto N.,
Sakurai I.,
Satoh K.
Publication year - 1993
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1993.33593255603.x
Subject(s) - testis determining factor , polymerase chain reaction , typing , medicine , immunology , human leukocyte antigen , microchimerism , graft versus host disease , lymphocyte , real time polymerase chain reaction , pathology , biology , gene , disease , y chromosome , antigen , genetics , pregnancy , fetus
A woman with recurrent Paget's disease of the vulva developed acute graft‐versus‐host disease (GVHD) 12 days after radical surgery and massive blood transfusion. Molecular diagnosis of lymphocyte chimerism in the peripheral blood was made by polymerase chain reaction (PCR) directed against a Y chromosome‐specific sex‐determining region Y (SRY) gene. PCR with skin biopsy after onset of GVHD also revealed infiltration of SRY‐positive donor lymphocytes. The diagnosis was confirmed by HLA‐DNA typing with PCR‐sequence‐specific oligonucleotide that revealed the presence of complex HLA‐DR chimerism in the peripheral lymphocytes collected after onset of GVHD. The use of SRY‐ directed PCR is a rapid technique for the early diagnosis of acute posttransfusion GVHD in female patients.