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Preparation of white‐cell‐poor blood components using a quadruple bag system
Author(s) -
Hirosue A.,
Yamamoto K.,
Shiraki H.,
Kiyokawa H.,
Maeda Y.,
Yoshinari M.
Publication year - 1988
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1988.28388219156.x
Subject(s) - buffy coat , centrifugation , platelet , chromatography , chemistry , andrology , white blood cell , red blood cell , blood cell , red cell , medicine , immunology , biochemistry
A method for the preparation of white‐cell‐poor red cells from 400 ml of blood collected in a quadruple bag with one 80‐ml and two 300‐ml satellite bags is described. In this procedure, a platelet concentrate was prepared from the buffy coat fraction obtained by the first centrifugation of whole blood. After centrifugation of whole blood for 5 minutes at 3500 × g , the plasma was transferred into the 300‐ml bag until the interface of red cells and plasma reached a level 32 mm from the top of the bag; then approximately 70 g of plasma and buffy coat were collected into the 80‐ml bag. The buffy coat fraction was centrifuged further for 5 minutes at 170 × g , and the supernatant (concentrated platelets in plasma) was transferred into the second 300‐ml bag. In this blood processing, the recovery of red cells into the packed red cells and of platelets into the platelet concentrate was 93 ± 4 percent and 52 ± 13 percent, respectively, of the original value. White cells in the packed red cells were 70 ± 28 × 10 7 with recovery of 32 ± 9 percent of the original value, and the lymphocytes in the white cells were only 7 ± 5 × 10 7 (7 ± 4% of the original value). White cell contamination of platelet concentrate was below the threshold of white cell detection by the microcell counter (less than 300 cells per μl of concentrate).