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INTER‐FAMILY USE OF MICROSATELLITE MARKERS IN LAMINARIALES: THE EXPERIENCE BETWEEN LAMINARIA DIGITATA AND LESSONIA SPP.
Author(s) -
Martínez E.A.,
Cardenas L.,
Figueroa C.,
Vidal R.,
Billot C.
Publication year - 2000
Publication title -
journal of phycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.85
H-Index - 127
eISSN - 1529-8817
pISSN - 0022-3646
DOI - 10.1046/j.1529-8817.1999.00001-136.x
Subject(s) - microsatellite , biology , laminaria digitata , genus , kelp , laminaria , algae , genetic marker , evolutionary biology , botany , zoology , genetics , allele , gene
Among the co‐dominant molecular markers, microsatellite loci have a number of advantages in population genetic studies. However, the different methods to hunt these markers are expensive, time consuming, and they require sophisticated laboratory equipment. Using in one species the microsatellites primers originally described for another one saves time and reduces costs. Examples in the literature revealed that microsatellites described for Gracilaria gracilis from France have not worked for other members of the Gracilariales. The results were not very promising, at least for Gracilaria chilensis from Chile. In this study, a number of microsatellite loci described for Laminaria digitata (Laminariaceae) from France were amplified and sequenced in two Lessonia (Lessoniaceae) species from Chile. Preliminary results show a partial conservation of both, flanking and tandem repeat regions. Some polymorphism has also been detected in Lessonia spp. The higher molecular affinity (conservation of primer sites) observed in species belonging to different families of brown algae, respect to that observed among species of the same genus in the red algae, is surprising. Such a result is strikingly counterintuitive when observing the morphological disparity among the Laminariales, respect to the similarity observed in the Gracilariales. It also reminds one of an earlier discussion by Gary Saunders about “When a family is not a family”.