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Prosaposin‐derived peptides enhanced sprouting of sensory neurons in vitro and induced sprouting at motor endplates in vivo
Author(s) -
Campana W. Marie,
Mohiuddin L.,
Misasi R.,
O'brien J.S.,
Calcutt N.A.
Publication year - 2000
Publication title -
journal of the peripheral nervous system
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 67
eISSN - 1529-8027
pISSN - 1085-9489
DOI - 10.1046/j.1529-8027.2000.00019.x
Subject(s) - sprouting , neurite , in vivo , in vitro , neurotrophin , sensory system , neurotrophic factors , neuroscience , microbiology and biotechnology , biology , chemistry , anatomy , biochemistry , receptor , botany
Abstract Prosaposin exhibits neurotrophic factor properties that are localized to a 12‐amino acid sequence located in the amino terminal portion of the saposin C domain. Prosaptides are peptides derived from the neurotrophic portion of prosaposin; these have been previously reported to be bioactive in neuroblastoma cell lines in vitro. We report that prosaptides were also bioactive in explants of adult primary sensory neurons by dose‐dependently increasing both the number (3‐ to 4‐fold) and elongation of these neurites by 50%. Local injection of prosaptides into the gluteus muscle of adult mice also induced sprouting at the motor endplate. Our results indicate that prosaptides are potent neuritogenic factors for both sensory and motor neurons of adult peripheral nerve.