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Tissue Engineered Artificial Skin Composed of Dermis and Epidermis
Author(s) -
Yang Eun Kyung,
Seo Young Kwon,
Youn Hee Hun,
Lee Doo Hoon,
Park Sue Nie,
Park Jung Keug
Publication year - 2000
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.2000.06334.x
Subject(s) - dermis , epidermis (zoology) , artificial skin , tissue engineering , skin repair , anatomy , biomedical engineering , biology , wound healing , medicine , immunology
We made an artificial skin comprised of a stratified layer of keratinocytes and a dermal matrix with a type I collagen containing fibroblasts. In this work, we showed keratinocyte behavior under primary culture, gel contractions varying with concentration of collagen solution, and cell growth plots in the collagen gel. The optimum behavior of dermal equivalent could be obtained using 3.0 mg/ml collagen solution and attached gel culture. The attached gel culture had a jumping effect of growth factor on cell growth at the lag phase. To develop the artificial skin, 1× 10 5 cells/cm 2 of keratinocytes were cultured on the dermal equivalent at air‐liquid interface. Finally, to overcome the problem that artificial skin of collagen gel was torn easily during suturing of grafting, we prepared histocompatible collagen mesh and attached the mesh to the bottom of the gel. Cultured artificial skins were successfully grafted onto rats.