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In Vitro Comparison Study of CD63 and CD62P Expression After Contacting Leukocyte Filters
Author(s) -
Fukunaga Ken,
Shimoyama Takashi,
Yamaji Ken,
Yamane Shingo,
Sueoka Akinori,
Nosé Yukihiko
Publication year - 1999
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1046/j.1525-1594.1999.06284.x
Subject(s) - polyester , flow cytometry , polyurethane , cd63 , platelet , platelet activation , materials science , chemistry , in vitro , filtration (mathematics) , biomedical engineering , chromatography , microbiology and biotechnology , composite material , immunology , biochemistry , medicine , biology , microrna , statistics , mathematics , microvesicles , gene
CD63 and CD62P have been recognized as platelet activation markers. This study investigated the secretion of these antigens to compare the platelet activation between a newly developed stainless steel leukocyte filter (SSLF) and 7 polyester or polyurethane commercially available leukocyte filters. Flow cytometry demonstrated that the SSLF initiated significantly smaller effects in terms of mean fluorescence intensity of CD63 (p < 0.03) and of the amount of CD62P expressing platelets (p < 0.002) compared to the polyurethane filters. However, there was no statistical difference between the SSLF and polyester filters. The result of this study suggests that the SSLF caused less platelet activation than the polyurethane filters and has biocompatible characteristics comparable to the currently available polyester filters. Stainless steel was selected because of its physicochemical conductivity. With these results, further evaluation of the SSLF will be continued in an attempt to develop an active immunomodulator using this unique characteristic.