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Transforming growth factor‐β2 (TGF‐β2) reverses the inhibitory effects of fibrin sealant on cutaneous wound repair in the pig
Author(s) -
Petratos Peter B.,
Felsen Diane,
Trierweiler Grant,
Pratt Bruce,
McPherson John M.,
Poppas Dix P.
Publication year - 2002
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1046/j.1524-475x.2002.10409.x
Subject(s) - sealant , fibrin , transforming growth factor , wound healing , inhibitory postsynaptic potential , pharmacology , chemistry , medicine , surgery , immunology , organic chemistry
Tensile strength of 2‐cm, full‐thickness, surgically incised porcine skin wounds sealed with fibrin sealant was enhanced compared to conventionally sutured wounds at 6 hours postwounding, but was significantly reduced after 3 days. Supplementation of fibrin sealant with transforming growth factor‐β2 (TGF‐β2) reversed the inhibitory effects of fibrin sealant on tensile strength at 3 days, and enhanced tensile strength at 7 days compared to suture or fibrin sealant alone. By 14 days, the tensile strengths of all wounds were similar, although wounds treated with fibrin sealant supplemented with TGF‐β2 showed a small, but statistically significant, improvement in wound strength compared to wounds treated with fibrin sealant alone. Histological assessment at day 7 revealed significant remnants of fibrin sealant at the wound site following fibrin sealant treatment alone, while wounds treated with fibrin sealant supplemented with TGF‐β2 or suture exhibited fibroblast infiltration and extracellular matrix deposition. At day 7, TGF‐β was immunolocalized in the base and margins of only wounds treated with fibrin sealant supplemented with TGF‐β2. A significant increase in matrix metalloproteinase‐9 activity was found in fibrin sealant–treated wounds at day 7 as compared to sutured wounds. Addition of TGF‐β to the fibrin sealant suppressed the up‐regulation of matrix metalloproteinase‐9 in these wounds. These results suggest that fibrin sealant supplemented with TGF‐β may provide superior wound healing as compared to fibrin sealant alone. (WOUND REP REG 2002;10:252–258)