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Improving heterologous gene expression in Aspergillus niger by introducing multiple copies of protein‐binding sequence containing CCAAT to the promoter
Author(s) -
Liu L.,
Liu J.,
Qiu R.X.,
Zhu X.G.,
Dong Z.Y.,
Tang G.M.
Publication year - 2003
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.2003.01321.x
Subject(s) - aspergillus niger , heterologous , biology , promoter , gene , microbiology and biotechnology , heterologous expression , gene expression , binding site , expression vector , activator (genetics) , caat box , genetics , biochemistry , recombinant dna
Aims: To increase the expression level of heterologous gene in Aspergillus niger . Methods and Results: Inserting multiple copies of an activator protein‐binding site originally encoded in the cis ‐regulatory region of A. niger glucoamylase gene ( glaA ), CCAAT‐containing sequence, into the promoter of an expression plasmid greatly enhanced the production of heterologous protein. Conclusion: The promoter activity was increased by the introduction of the CCAAT‐containing sequence resulting in increased gene expression. Significance and Impact of the Study: This study provides evidence that the CCAAT‐containing region of the glaA promoter of A. niger is a binding site for a positive transcriptional factor and demonstrates the possibility for improving the expression of both heterologous and endogenous genes in Aspergillus .
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