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Pulsed‐field gel electrophoresis typing of Hafnia alvei isolated from chub‐packed and retail ground beef
Author(s) -
Gamage,
Luchansky,
Ingham
Publication year - 1998
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1998.00087.x
Subject(s) - pulsed field gel electrophoresis , ribotyping , biology , microbiology and biotechnology , typing , gel electrophoresis , polymerase chain reaction , genotype , genetics , gene
Thirty‐eight isolates of Hafnia alvei were characterized by biochemical profiles, ribotyping and pulsed‐field gel electrophoresis (PFGE) patterns. The isolates were recovered from chub‐packed (19 isolates) or retail (nine isolates) ground beef, or were obtained from culture repositories (10 isolates). Biochemical profiling differentiated the 38 isolates into five groups and a commercial ribotyping method recognized 11 groups, whereas PFGE differentiated the same 38 isolates into 19 groups. These data substantiate that PFGE is a highly discriminatory tool for establishing the relatedness among Hafnia alvei strains.