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Studies in polysaccharide production and growth of Azotobacter vinelandii MTCC 2459, a plant rhizosphere isolate
Author(s) -
Vermani M.V.,
Kelkar S.M.,
Kamat M. Y.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1046/j.1472-765x.1997.00137.x
Subject(s) - azotobacter vinelandii , rhizosphere , azotobacter , division (mathematics) , fermentation , research centre , biology , microbiology and biotechnology , library science , food science , mathematics , nitrogen fixation , computer science , bacteria , nitrogenase , genetics , arithmetic
Azotobacter vinelandii MTCC 2459, a novel exopolysaccharide (EPS) producer, was isolated from Nelumbium nelumbo (lotus) stem. The EPS produced has a sugar composition different from the existing industrial gums (glucose, galactose and rhamnose). Optimization of the basal medium composition and other physical parameters (viz. pH, temperature, time, etc.) are reported in this paper. A final yield of 16·5% of the amount of the carbon source supplied was obtained in shake flask cultures, indicating the potential of the organism for production of useful EPS on a large (industrial) scale.