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3′,5′‐Cyclic Guanosine Monophosphate Activates Mitogen‐Activated Protein Kinase in Rat Pinealocytes
Author(s) -
Ho A. K.,
Hashimoto K.,
Chik C. L.
Publication year - 1999
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1046/j.1471-4159.1999.0730598.x
Subject(s) - protein kinase a , pinealocyte , zaprinast , cgmp dependent protein kinase , cyclic guanosine monophosphate , pde10a , mapk/erk pathway , phosphodiesterase 3 , medicine , mitogen activated protein kinase kinase , protein kinase inhibitor , endocrinology , kinase , mitogen activated protein kinase , chemistry , phosphodiesterase , phosphodiesterase inhibitor , microbiology and biotechnology , biology , biochemistry , pineal gland , melatonin , nitric oxide , enzyme
Abstract : The role of 3′,5′‐cyclic guanosine monophosphate (cGMP) in the activation of mitogen‐activated protein kinases (MAPKs) was investigated in rat pinealocytes. Treatment with dibutyryl cGMP (DBcGMP) dosedependently increased the phosphorylation of both p44 and p42 isoforms of MAPK. This effect of DBcGMP was abolished by PD98059 (a MAPK kinase inhibitor), H7 (a nonspecific protein kinase inhibitor), and KT5823 [a selective cGMP‐dependent protein kinase (PKG) inhibitor]. Elevation of cellular cGMP content by treatment with norepinephrine, zaprinast (a cGMP phosphodiesterase inhibitor), or nitroprusside was effective in activating MAPK. Natriuretic peptides that were effective in elevating cGMP levels in this tissue were also effective in activating MAPK. Our results indicate that, in this neuroendocrine tissue, the cGMP/PKG signaling pathway is an important mechanism used by hormones and neurotransmitters in activating MAPK.