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Identification of the genetic variants of κ‐casein in milk by isoelectric focusing electrophoresis
Author(s) -
Molina L H,
Casanova M,
Gonzalez L A,
Pinto M,
Carrasco E,
Brito C
Publication year - 2003
Publication title -
international journal of dairy technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.061
H-Index - 53
eISSN - 1471-0307
pISSN - 1364-727X
DOI - 10.1046/j.1471-0307.2003.00102.x
Subject(s) - isoelectric point , casein , isoelectric focusing , breed , genetic variants , electrophoresis , bovine milk , chromatography , milk protein , biology , chemistry , food science , microbiology and biotechnology , zoology , biochemistry , genotype , enzyme , gene
The genetic variants of κ‐casein (κ‐CN) A and B were analysed in milk from Holstein–Friesian (HF) and Jersey cows by means of isoelectric focusing (IEF) electrophoresis. Milk samples were obtained in triplicate from pure‐breed HF and Jersey cows (three of each) to estimate the protein content, casein and purify κ‐CN. The protein and casein contents in the milk from both breeds were statistically different (P < 0.05). The κ‐CN A migrates first with an isoelectric point (pI) of 7.2–7.3 and then B with a pI of 7.5–7.7. Differences in the expression proportion of both variants were detected.