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Eosinophilic major basic protein and interleukin‐5 in eosinophilic myositis
Author(s) -
Murata K.,
Sugie K.,
Takamure M.,
Fujimoto T.,
Ueno S.
Publication year - 2003
Publication title -
european journal of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 124
eISSN - 1468-1331
pISSN - 1351-5101
DOI - 10.1046/j.1468-1331.2003.00481.x
Subject(s) - myositis , medicine , eosinophilic , interleukin 5 , messenger rna , pathology , major basic protein , interleukin , reverse transcriptase , immunology , eosinophil , polymerase chain reaction , biology , cytokine , gene , biochemistry , asthma
To determine whether eosinophils play a critical role in muscle fiber damage in patients with eosinophilic myositis (EM). We investigated expression of eosinophilic major basic protein (MBP) and interleukin (IL)‐5 at the protein and mRNA levels in muscle biopsies from three patients with idiopathic EM. MBP deposits were found on the surface of eosinophils and muscle fibers surrounded by the eosinophils. Reverse transcriptase‐polymerase chain reaction analysis showed increased IL‐5 expression in EM muscle but not in control muscle. These results suggest that IL‐5 induces local accumulation of eosinophils and their release of MBP. The secreted proteins adhere to the muscle fiber membrane, resulting in muscle damage.