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Misexpression of genes in brain vesicles by in ovo electroporation
Author(s) -
Nakamura Harukazu,
Watanabe Yuji,
Funahashi Junichi
Publication year - 2000
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.2000.00501.x
Subject(s) - electroporation , in ovo , transfection , biology , microbiology and biotechnology , vesicle , embryo , gene , genetics , membrane
Transfection to living chick embryos in ovo by electroporation has been recently developed. In this mini‐review, misexpression in brain vesicles is introduced. To transfect, expression plasmid is inserted in the brain vesicle, and the square pulse of 25 V, 50 ms was charged five times. The translation product of the transfected gene is detected 2 h after electroporation, and reaches the peak at 24 h after electroporation. Transfection is so effective that this method is contributing greatly to the study of the molecular mechanisms of morphogenesis.