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Gene transfer into chicken embryos as an effective system of analysis in developmental biology
Author(s) -
Yasugi Sadao,
Nakamura Harukazu
Publication year - 2000
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1046/j.1440-169x.2000.00500.x
Subject(s) - biology , electroporation , gene transfer , embryo , developmental biology , embryology , gene , animal model , computational biology , microbiology and biotechnology , genetics , endocrinology
Chicken embryos have been used as a model animal in developmental biology since the time of comparative and experimental embryology. Recent application of gene transfer techniques to the chicken embryo increases their value as an experimental animal. Today, gene transfer into chicken cells is performed by three major systems, lipofection, electroporation and the virus‐mediated method. Each system has its own features and applicability. In this overview and the associated four minireviews, the methods and application of each system will be presented.