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Enhanced Detection of Prune Dwarf Virus in Peach Leaves by Immunocapture‐Reverse Transcription‐Polymerase Chain Reaction with Nested Polymerase Chain Reaction (IC‐RT‐PCR Nested PCR)
Author(s) -
HELGUERA P. R.,
DOCAMPO D. M.,
NOME S. F.,
DUCASSE D. A.
Publication year - 2002
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1046/j.1439-0434.2002.00696.x
Subject(s) - nested polymerase chain reaction , biology , polymerase chain reaction , reverse transcription polymerase chain reaction , reverse transcriptase , microbiology and biotechnology , virology , real time polymerase chain reaction , inverse polymerase chain reaction , gene , genetics , messenger rna
To improve the sensitivity of detection of Prune dwarf virus (PDV) in peach trees, the immunocapture‐reverse transcription‐polymerase chain reaction combined with nested PCR (IC‐RT‐PCR nested PCR) was modified. The nested PCR substantially improved PDV detection compared with double antibody sandwich‐ELISA. Both assays use the same tissue extracts.