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Determination of the formation of the stratum corneum reservoir for two different corticosteroid formulations using tape stripping combined with UV/VIS spectroscopy
Author(s) -
Pelchrzim Ragna v.,
Weigmann HansJürgen,
Schaefer Hans,
Hagemeister Timo,
Linscheid Michael,
Shah Vinod P.,
Sterry Wolfram,
Lademann Jürgen
Publication year - 2004
Publication title -
jddg: journal der deutschen dermatologischen gesellschaft
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.463
H-Index - 60
eISSN - 1610-0387
pISSN - 1610-0379
DOI - 10.1046/j.1439-0353.2004.04796.x
Subject(s) - stratum corneum , penetration (warfare) , clobetasol propionate , chemistry , corneocyte , chromatography , dermatology , medicine , mathematics , pathology , psoriasis , operations research
Summary Background: The stratum corneum, the outermost layer of the skin, not only represents a barrier, but also a reservoir for topically applied substances. Both properties influence the penetration process, as does the vehicle. It determines the penetration pathway and the retention time of the topically applied substances. Methods: Two commercial products (Temovate Cream and Temovate Emollient), both containing 0.05 % clobetasol propionate, were applied on the inner forearms of healthy volunteers. 20 tape strips were removed from each treated skin area. The horny layer profile was determined measuring the absorption of the corneocytes at 430 nm. The concentration of clobetasol propionate on the individual tapes was then analyzed. Results: We found differences in the reservoir formation depending on the formulation applied, which correlated to the biological response in the form of a blanching effect. Conclusions: The method applied is well‐suited to determine the reservoir formation in the stratum corneum for topically applied substances.