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Labelling of Rat Endothelial Cells with Antibodies to vWF, RECA‐1, PECAM‐1, ICAM‐1, OX‐43 and ZO‐1
Author(s) -
ÜLGER H.,
KARABULUT A. K.,
PRATTEN M. K.
Publication year - 2002
Publication title -
anatomia, histologia, embryologia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.34
H-Index - 35
eISSN - 1439-0264
pISSN - 0340-2096
DOI - 10.1046/j.1439-0264.2002.00357.x
Subject(s) - yolk sac , endothelial stem cell , endothelium , biology , embryonic stem cell , microbiology and biotechnology , pathology , embryo , endocrinology , in vitro , medicine , biochemistry , gene
Labelling with endothelium specific monoclonal antibodies, von Willebrand Factor (vWF), rat endothelial cell antigen‐1 (RECA‐1), platelet‐endothelial cell adhesion molecule‐1 (PECAM‐1), intercellular adhesion molecule‐1 (ICAM‐1), OX‐43 and zonula occludentes‐1 (ZO‐1), was investigated in cryostat sections of vessels from rats of different ages using a confocal microscope. The results showed that labelling of the vWF was positive in endothelial cells from adult, fetal and different ages of embryonic rat. Labelling with RECA‐1 was weakly positive in adult rat aorta and lung endothelial cells but not in embryonic yolk sac endothelial cells. Labelling using PECAM‐1, ICAM‐1 and OX‐43 was negative in both adult and embryonic endothelial cells. ZO‐1 showed positive but very weak reactivity in embryonic yolk sac endothelial cells. The expression of vWF on vessels from adult and 19.5‐day fetal tissues was strongly positive. However, the expression of vWF in embryonic endothelial cells was dependent on the gestational age. While the 11.5‐day yolk sac vessels stained weakly, staining gradually increased in 13.5‐, 15.5‐ and 17.5‐day‐old yolk sac vessels. The results suggest that vWF is a reliable endothelial cell marker in rat vascular endothelial cells, including both fetal and embryonic stages.