Open AccessThe structure of the carbohydrate backbone of the lipopolysaccharides from Bordetella hinzii and Bordetella bronchiseptica
Author(s) -
Vinogradov Evgeny
Publication year - 2000
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.2000.01512.x
Subject(s) - bordetella bronchiseptica , bordetella , chemistry , microbiology and biotechnology , hydrolysis , bacteria , biochemistry , bordetella pertussis , biology , genetics
The structure of the core‐lipid A region of the lipopolysaccharides from Bordetella hinzii and Bordetella bronchiseptica has been analyzed. Lipopolysaccharides were deacylated using strong alkaline hydrolysis, the products were separated by high performance anion‐exchange chromatography and analyzed by NMR and mass spectrometry. The following structure of the products can be deduced from the experimental results: where for the product from Bordetella hinzii N = H, R = H, β‐FucN4N‐ or partially N ‐acetylated Sug‐(1–3)‐β‐FucN4N and for the product from Bordetella bronchiseptica N = α‐Hep, R = H, β‐FucN4N, β‐FucN4NMe or partially N ‐acetylated Sug‐(1–3)‐β‐FucN4N or Sug‐(1–3)‐β‐FucN4NMe; Sug = 2,3‐diamino‐2,3,4‐trideoxy‐hex‐4‐enuronopyranosyl.