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Fusion genetic analysis of gibberellin signaling mutants
Author(s) -
Raventos Dora,
Meier Carsten,
Jensen Ole Mattsson anders B.,
Mundy John
Publication year - 2000
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2000.00759.x
Subject(s) - gibberellin , mutant , biology , computational biology , genetics , microbiology and biotechnology , gene
Summary A fusion genetic strategy was used to identify gibberellin (GA) signaling mutants in transgenic Arabidopsis expressing the β‐glucuronidase ( GUS ) and firefly luciferase ( LUC ) reporter genes under control of the GA‐responsive GASA1 promoter. Initial analyses determined the spatial and temporal patterns of reporter expression, and showed that reporter induction by GA was antagonized by ABA. γ‐Irradiated M 2 progeny with altered reporter activities were identified by LUC bioimaging followed by GUS assays and northern hybridization of the endogenous GASA1 mRNA. Genetic analysis showed that three mutants, which overexpressed both reporters and endogenous GASA1 , were caused by recessive ( goe1 and goe2 , for GASA over‐expressed) and semi‐dominant ( goe3 ) mutations at different loci. These mutants are altered in their sensitivity to GA and the GA biosynthetic inhibitor paclobutrazol, and in the expression of several GA signaling related genes.