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Use of the IGF BAC library for physical mapping of the Arabidopsis thaliana genome
Author(s) -
Mozo Teresa,
Fischer Sabine,
MeierEwert Sebastian,
Lehrach Hans,
Altmann Thomas
Publication year - 1998
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1998.00299.x
Subject(s) - arabidopsis thaliana , physical mapping , genome , biology , computational biology , genetics , gene , gene mapping , mutant , chromosome
Summary In order to generate a physical map of theArabidopsis thalianagenome based on bacterial artificial chromosome clones (BACs), an iterative high throughput hybridisation strategy was applied and its efficiency was evaluated. Thus, probes generated from both ends of 500 BAC clones selected from theArabidopsis–IGF–BAC library were hybridised to the entire library gridded on high density filters. The 1000 hybridisation reactions identified 4496 clones (41.8% of the complete library, or 50.3% if organellar, centromeric, and ribosomal DNA carrying clones are excluded) which were assembled into a minimum of 220 contigs. These results demonstrate the viability of the applied ‘double‐end clone‐limited/sampling without replacement’ hybridisation strategy for the generation of a high resolution physical map, and provide a highly useful resource for map‐based gene cloning approaches and further genome analysis.