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Regulation of tylosin production and morphological differentiation in Streptomyces fradiae by TylP, a deduced γ ‐butyrolactone receptor
Author(s) -
Stratigopoulos George,
Gandecha Atul R.,
Cundliffe Eric
Publication year - 2002
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1046/j.1365-2958.2002.03044.x
Subject(s) - tylosin , streptomyces fradiae , biology , repressor , transcription (linguistics) , promoter , gene , streptomyces , activator (genetics) , transcription factor , gene expression , microbiology and biotechnology , genetics , actinomycetales , bacteria , antibiotics , linguistics , philosophy
Summary During promoter‐probe analysis carried out in Streptomyces lividans , the TylP protein powerfully inhibited reporter gene expression from the tylP promoter, raising the likelihood that tylP is autoregulated in its native host, Streptomyces fradiae . Also in S. lividans , TylP negatively controlled the tylQ promoter, even though tylQ could still be switched off in S. fradiae strains specifically disrupted in tylP . Under the latter conditions, tylosin production was brought forward and enhanced, whereas overexpression of tylP re‐sulted in reduced levels of the antibiotic, accompanied by barely detectable transcription from multiple genes of the tylosin biosynthetic cluster. Unexpectedly, overexpression of tylP reduced transcription of tylS , a transcriptional activator essential for tylosin production. This was probably a direct effect, as TylP also reduced expression from the tylS promoter in S. lividans . For these several reasons, we conclude that TylP acts as a repressor during tylosin biosynthesis. In addition, TylP influences morphological differentiation in S. fradiae . On solid media, strains in which tylP was disrupted sporulated significantly earlier than wild type and, in liquid culture, displayed hyperfragmentation.