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Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR‐RFLP
Author(s) -
Fanello C.,
Santolamazza F.,
Della Torre A.
Publication year - 2002
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1046/j.1365-2915.2002.00393.x
Subject(s) - biology , anopheles gambiae , sibling species , sensu , restriction fragment length polymorphism , sensu stricto , vector (molecular biology) , identification (biology) , larva , species complex , species identification , zoology , malaria , polymerase chain reaction , genetics , ecology , gene , immunology , genus , recombinant dna , phylogenetic tree
For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto , we describe a PCR‐RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large‐scale processing of field‐collected larval and adult specimens to be identified in malaria vector studies.