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Increased sodium–proton antiporter activity in patients with obstructive sleep apnoea
Author(s) -
Tepel Martin,
Sanner Bernd M.,
Van Der Giet Marcus,
Zidek Walter
Publication year - 2000
Publication title -
journal of sleep research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.297
H-Index - 117
eISSN - 1365-2869
pISSN - 0962-1105
DOI - 10.1046/j.1365-2869.2000.00207.x
Subject(s) - antiporter , sodium–hydrogen antiporter , intracellular ph , sodium , chemistry , medicine , sleep (system call) , endocrinology , intracellular , biochemistry , computer science , operating system , organic chemistry
Cytosolic pH (pH i ) and the activity of the sodium–proton antiporter (Na + /H + antiporter) were measured in lymphocytes from 22 patients with obstructive sleep apnoea and from 24 age‐matched healthy subjects (Controls). The cellular Na + /H + antiporter was measured spectrophotometrically using a pH‐sensitive fluorescent dye after intracellular acidification using sodium propionate. Resting pH i was similar in lymphocytes from patients with obstructive sleep apnoea and from controls (7.36 ± 0.20, n =22; vs. 7.35 ± 0.19, n =24; mean ± SD). The Na + /H + antiporter activity was significantly higher in patients with obstructive sleep apnoea than in controls (11.87 ± 3.26 × 10 –3 pH i /s vs. 4.38 ± 1.40 × 10 –3 pH i /s; P < 0.0001). The apparent affinity of the Na + /H + antiporter was not significantly different between the groups (6.90 ± 0.23 vs. 6.87 ± 0.20). In patients with obstructive sleep apnoea the activity of the Na + /H + antiporter remained stable during the night. The activity of the Na + /H + antiporter was 13.49 ± 4.80 × 10 –3 pH i /s at 20.00 and 13.26 ± 6.13 × 10 –3 pH i /s at 02.00. From the present results it is concluded that an increased cellular Na + /H + antiporter activity may be a genetic marker for patients who are predisposed to obstructive sleep apnoea.