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Ghrelin Stimulates Insulin Secretion from the Pancreas of Normal and Diabetic Rats
Author(s) -
Adeghate E.,
Ponery A. S.
Publication year - 2002
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1046/j.1365-2826.2002.00811.x
Subject(s) - medicine , endocrinology , ghrelin , yohimbine , insulin , pancreas , population , receptor antagonist , diabetes mellitus , antagonist , receptor , environmental health
Abstract Ghrelin is a novel 28‐amino acid gut–brain peptide, which was first isolated in the rat stomach. This study examined the effect of ghrelin on insulin secretion from the isolated pancreas of normal and diabetic rats. Diabetes was induced by a single dose of streptozotocin. Four weeks after the induction of diabetes, pancreatic tissue fragments of normal and diabetic rats were treated with different concentrations (10 −12 , 10 −9 and 10 −6 M) of ghrelin. Ghrelin evoked large and significant increases in insulin secretion from the pancreas of both normal and diabetic rats. In the pancreas of normal rats, diltiazem (calcium channel antagonist) or a combination of atropine (muscarinic cholinergic receptor antagonist), propranolol (β‐adrenergic receptor antagonist) and yohimbine (α 2 ‐adrenergic receptor antagonist) significantly reduced the stimulatory effect of ghrelin on insulin secretion. Diltiazem and yohimbine failed to inhibit ghrelin‐evoked insulin release in diabetic rat pancreas. Ghrelin‐immunoreactivity cells was observed in 2.6% and 3.8% of the total cell population in the islet of Langerhans of normal and diabetic rats, respectively.