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Pharmacological Characterization of Oxytocin‐Binding Sites in Rat Spinal Cord Membranes: Comparison with Embryonic Cultured Spinal Cord Neurones and Astrocytes
Author(s) -
Evrard M. E.,
Strosser M. T.,
Di ScalaGuenot D.
Publication year - 1997
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1046/j.1365-2826.1997.00614.x
Subject(s) - spinal cord , astrocyte , immunocytochemistry , central nervous system , biology , glial fibrillary acidic protein , receptor , oxytocin , microbiology and biotechnology , medicine , endocrinology , neuroscience , immunohistochemistry , biochemistry , immunology
Detection and pharmacological characterization of OT‐binding sites were performed on 12‐day‐old rat spinal cord membranes and on embryonic cultured spinal neurones and astrocytes after 12 days in culture . In neurone‐enriched cultures, OT‐binding sites were detected by autoradiography on cells morphologically comparable to neurone‐specific enolase immunoreactive cells. In astrocyte cultures, as shown by combination of autoradiography and immunocytochemistry, OT‐binding sites were detected on cells expressing the glial fibrillary acidic protein (a specific astrocytic marker).