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Evaluation of autofocus functions in molecular cytogenetic analysis
Author(s) -
SANTOS A.,
ORTIZ DE SOLÓRZANO C.,
VAQUERO J. J.,
PEÑA J. M.,
MALPICA N.,
DEL POZO F.
Publication year - 1997
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1046/j.1365-2818.1997.2630819.x
Subject(s) - autofocus , figure of merit , image (mathematics) , computer science , function (biology) , fluorescence , in situ , artificial intelligence , computer vision , pattern recognition (psychology) , biological system , optics , physics , biology , evolutionary biology , focus (optics) , meteorology
This work describes a systematic evaluation of several autofocus functions used for analytical fluorescent image cytometry studies of counterstained nuclei. Focusing is the first step in the automatic fluorescence in situ hybridization analysis of cells. Thirteen functions have been evaluated using qualitative and quantitative procedures. For the last of these procedures a figure‐of‐merit (FOM) is defined and proposed. This new FOM takes into account five important features of the focusing function. Our results show that functions based on correlation measures have the best performance for this type of image.
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