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Occurrence and levels of phages proposed as surrogate indicators of enteric viruses in different types of sludges
Author(s) -
Lasobras J.,
Dellunde J.,
Jofre J.,
Lucena F.
Publication year - 1999
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1999.00722.x
Subject(s) - enteric virus , enteric bacteria , microbiology and biotechnology , biology , feces , escherichia coli , genetics , gene
A method based on the treatment of sludge with beef extract recovered, with similar efficiency, the three groups of bacteriophages studied from different kinds of sludges. The three groups of bacteriophages were found in high numbers in the different sludge types, the highest value being that of somatic coliphages in primary sludge of a biological treatment plant (1·1 × 10 5 pfu g −1 ) and the lowest being that of Bacteroides fragilis phages (110 pfu g −1 ) in de‐watered, anaerobically, mesophilically‐digested sludge. All phages studied accumulated in the sludges. In primary and activated sludges, all three types accumulated similarly but in lime‐treated sludge and de‐watered, anaerobically, mesophilically‐digested sludge, the relative proportion of F‐specific bacteriophages decreased significantly with respect to somatic coliphages and bacteriophages infecting B. fragilis . All phages survived successfully in stored sludge, depending on the temperature, and again, F‐specific bacteriophages survived less successfully than the others.