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Note: Molecular cloning of chitinase genes from Vibrio anguillarum and V. parahaemolyticus
Author(s) -
Hirono,
; Yamashita,
Marcelo Saldanha Aoki
Publication year - 1998
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1046/j.1365-2672.1998.00439.x
Subject(s) - vibrio anguillarum , vibrio parahaemolyticus , biology , chitinase , gene , molecular cloning , vibrio alginolyticus , microbiology and biotechnology , vibrio harveyi , open reading frame , escherichia coli , dna , genetics , vibrio , peptide sequence , bacteria
Chitinase genes from Vibrio anguillarum KV9001 and V. parahaemolyticus ATCC17802 were cloned into Escherichia coli. Open reading frames of chitinase genes from V. anguillarum (vac) and V. parahaemolyticus (vpc) are 1755 bp and 1890 bp, respectively. The deduced amino acid sequences of these genes have 71·6% identity. There are two consensus sequence regions in the VAC and VPC proteins. The vac gene was highly prevalent in V. anguillarum , and the DNA probe of the vac gene hybridized to V. alginolyticus and Beneckea proteolytica DNA. The DNA probe of the vpc gene hybridized to V. alginolyticus, V. harveyi and V. ordalii DNA.