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Juvenile hormone controls early trypsin gene transcription in the midgut of Aedes aegypti
Author(s) -
Noriega F. G.,
Shah D. K.,
Wells M. A.
Publication year - 1997
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1997.00154.x
Subject(s) - midgut , biology , trypsin , aedes aegypti , transcription (linguistics) , blood meal , gene , microbiology and biotechnology , transcription factor , protease , biochemistry , enzyme , linguistics , botany , philosophy , food science , larva
Early trypsin is a female‐specific protease present in theAedes aegyptimidgut during the first few hours after ingestion of a blood meal. The enzymatic activity of early trypsin plays an essential role in the transcriptional activation of the late trypsin gene, which encodes the major midgut endoprotease involved in blood meal protein digestion. Transcription of the early trypsin gene is part of the normal post‐emergence maturation of the midgut in the adult female. Abdominal ligation within 1 h of emergence completely prevented the transcription of the early trypsin gene. Topically applied JH III or methoprene induced transcription of the early trypsin gene in ligated abdomens to levels similar to those observed in non‐ligated females. The induction of early trypsin transcription by JH is dose‐dependent and ‘head‐independent’, suggesting that factors coming from the neurosecretory axis are not required.