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Detection of antigen‐specific cytotoxic T lymphocytes: significance for investigative dermatology
Author(s) -
Ogg G. S.
Publication year - 2000
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1046/j.1365-2230.2000.00651.x
Subject(s) - dermatopathology , human leukocyte antigen , flow cytometry , cytotoxic t cell , antigen , cd8 , immunology , disease , t lymphocyte , biology , medicine , pathology , in vitro , genetics
Novel recombinant tetrameric complexes of HLA class I molecules allow the direct visualization of antigen‐specific CD8+ T cells using flow cytometry. By facilitating the quantification, isolation and phenotypic analysis of CD8+ T cells, the use of HLA tetramers has extended our understanding of the role of cellular immunity in various disease settings. Recently the technique has also been applied to the study of cutaneous disease and provides insights into mechanisms of dermatopathology.