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A rapid and specific whole blood HPA‐1 phenotyping by flow cytometry using two commercialized monoclonal antibodies directed against GP IIIa and GP IIb‐IIIa complexes
Author(s) -
Sorel Nathalie,
Brabant Severine,
Christiaens Luc,
Brizard Andre,
Mauco Gerard,
Macchi Laurent
Publication year - 2004
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2003.04757.x
Subject(s) - flow cytometry , monoclonal antibody , neonatal alloimmune thrombocytopenia , platelet , immunology , thrombocytopenic purpura , antibody , monoclonal , antigen , platelet membrane glycoprotein , medicine , immune system , alloimmunity , whole blood , microbiology and biotechnology , biology , pregnancy , fetus , genetics
Summary The human platelet antigen 1 (HPA‐1) system has been implicated in rare but severe diseases, such as neonatal alloimmune thrombocytopenic purpura, post‐transfusion purpura and immune platelet refractoriness. We developed a flow cytometry assay for HPA‐1 phenotyping using two commercial monoclonal antibodies, P2 and SZ21, directed against glycoprotein (GP) IIb–IIIa and GP IIIa respectively. One hundred and twenty‐seven healthy controls were studied and ratios of mean fluorescence intensity for P2 and SZ21 discriminated between HPA‐1a homozygotes and heterozygotes. These two monoclonal antibodies, coupled with flow cytometry represent a rapid and reliable tool for platelet HPA‐1 typing to aid diagnosis.