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Hypermethylation of the M27β (DXS255) locus in chronic B‐cell leukaemia
Author(s) -
Crossen Peter E.,
Morrison Mary J.
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.00540.x
Subject(s) - hpaii , locus (genetics) , dna methylation , methylation , cancer research , medicine , pathology , biology , microbiology and biotechnology , immunology , dna , gene , genetics , gene expression
We have investigated the methylation status of the M27β (DXS255) locus in 21 female patients with chronic B‐cell leukaemia and in 20 normal controls. DNA was digested with PstI and then with the methylation sensitive enzyme HpaII and probed with the M27β probe. Eight patients (38%) showed hypermethylation of the M27β locus which was not seen in any of the normal controls. Hypermethylation of the M27β locus has also been found in acute myeloid leukaemia, acute lymphocytic leukaemia and lymphoma, suggesting that hypermethylation of the M27β locus is associated with the leukaemic process.