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Convenient genotyping of six myostatin mutations causing double‐muscling in cattle using a multiplex oligonucleotide ligation assay
Author(s) -
Karim L,
Coppieters W,
Grobet L,
Georges M,
Valentini A
Publication year - 2000
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1046/j.1365-2052.2000.00684.x
Subject(s) - genotyping , biology , multiplex , oligonucleotide , myostatin , genetics , multiplex polymerase chain reaction , microbiology and biotechnology , ligation , multiplex ligation dependent probe amplification , gene , phenotype , genotype , polymerase chain reaction , exon
We herein describe a procedure that allows for simultaneous genotyping of six loss‐of‐function mutations in the bovine myostatin gene associated with the double‐muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.