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Distribution of transcripts for the brain‐specific GDP/GTP exchange factor collybistin in the developing mouse brain
Author(s) -
Kneussel M.,
Engelkamp D.,
Betz H.
Publication year - 2001
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.0953-816x.2000.01411.x
Subject(s) - gephyrin , pleckstrin homology domain , guanine nucleotide exchange factor , microbiology and biotechnology , biology , gtpase , alternative splicing , cdc42 , glycine receptor , gene isoform , genetics , signal transduction , gene , glycine , amino acid
Abstract The dbl‐like GDP/GTP exchange factor, collybistin, binds to the receptor anchoring protein gephyrin and activates the Rho‐like GTPase Cdc42. Collybistin was found in two splice variants I and II, both of which share a tandem Dbl homology/pleckstrin homology (DH/PH) domain. In heterologous expression systems, collybistin II induces the formation of submembraneous gephyrin aggregates and therefore has been implicated in inhibitory synapse formation. Expression of collybistin is restricted to neuronal tissues and is predominantly found in brain. Here, we investigated the spatio‐temporal distribution of collybistin transcripts in the embryonic mouse brain and compared it to gephyrin and glycine receptor mRNA patterns. Our data show that collybistin expression is upregulated when neurons become postmitotic and start to differentiate.