Open AccessTumour‐related enzyme alterations in the clear cell type of human renal cell carcinoma identified by two‐dimensional gel electrophoresis
Author(s) -
Balabanov Stefan,
Zimmermann Uwe,
Protzel Chris,
Scharf Christian,
Klebingat KlausJürgen,
Walther Reinhard
Publication year - 2001
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.0014-2956.2001.02546.x
Subject(s) - aldehyde dehydrogenase , renal cell carcinoma , enzyme , proteome , dehydrogenase , cell , gel electrophoresis , two dimensional gel electrophoresis , biochemistry , kidney , carcinoma , biology , microbiology and biotechnology , chemistry , pathology , proteomics , medicine , endocrinology , gene
To identify tumour‐related enzyme alterations we have used 2D‐gels to analyse the proteome from dissected malignant and benign kidney areas from patients with clear‐cell‐type renal carcinoma. The expression of 12 proteins was diminished in tumour. Four proteins were characterized by mass spectrometry and were identified as enoyl‐CoA hydratase, α‐glycerol‐3‐phosphate dehydrogenase, aldehyde dehydrogenase 1 and aminoacylase‐I.