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Role of leukaemia inhibitory factor in the induction of pluripotent stem cells in mice
Author(s) -
Xu Jie,
Wang Feng,
Tang Zhouping,
Zhan Yanqiang,
Zhang Jinping,
Yan Qiuyue,
Xiong Yongjie,
Xie Xuewei,
Wu Jun,
Zhang Suming
Publication year - 2010
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1042/cbi20090484
Subject(s) - reprogramming , induced pluripotent stem cell , leukemia inhibitory factor , somatic cell , microbiology and biotechnology , inhibitory postsynaptic potential , embryonic stem cell , biology , stem cell , alkaline phosphatase , mechanism (biology) , chemistry , cell , biochemistry , neuroscience , gene , enzyme , physics , quantum mechanics
iPS (induced pluripotent stem) cells can be induced from somatic cells in mice by genetic manipulation. Most previously established mouse iPS cell lines have been derived using feeder layers supplemented with exogenous LIF (leukaemia inhibitory factor). Although a feeder‐free induction system has been developed in recent studies, LIF is still required for reprogramming, but its role in the generation of mouse iPS cells has remained elusive. In this study, we investigated its contribution to the induction of pluripotency. Our results showed that LIF activates AP (alkaline phosphatase) through a c‐Myc‐dependent mechanism. Moreover, it acts as a protective factor during the transition from AP‐positive colonies to Oct3/4‐positive cells. These findings illustrate a mechanism by which LIF may integrate signalling into reprogramming.