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Growth and production of cholesterol oxidase by alginate‐immobilized cells of Rhodococcus equi No. 23
Author(s) -
Chang YuChih,
Chou ChengChun
Publication year - 2002
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1042/ba20010058
Subject(s) - calcium alginate , population , rhodococcus equi , chromatography , incubation , chemistry , bead , stationary phase , biochemistry , biology , calcium , materials science , demography , organic chemistry , sociology , virulence , composite material , gene
Rhodococcus equi No. 23 was immobilized in calcium alginate. No detrimental effect on the viability of the test organism was observed during the immobilization procedure. Approx. 98% of the cell population originally present in the alginate solution were immobilized in the gel beads. When the cells of an equal volume of the culture, obtained respectively at exponential phase (12 h preculture), late‐exponential phase (20 h preculture) or stationary phase (36 h preculture) were immobilized, the gel beads prepared with the stationary‐phase culture were found to contain the highest cell population [about 10 8 colony‐forming units (CFU)/g of beads]. In addition, gel beads, prepared with late‐exponential‐phase culture, exhibited the highest production of cholesterol oxidase (CholOx) after 48 h of incubation. Increasing the bead mass from 3.5 to 14.0 g/100 ml of medium increased CholOx production. However, further increasing the bead mass resulted in a reduction of CholOx production. Furthermore, on the basis of a similar initial cell population, the alginate‐immobilized cells of R. equi No. 23 produced a significantly higher amount of CholOx ( P <0·05) than did the free cells.