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Identification of novel functional TBP‐binding sites and general factor repertoires
Author(s) -
Denissov Sergey,
van Driel Marc,
Voit Renate,
Hekkelman Maarten,
Hulsen Tim,
Hernandez Nouria,
Grummt Ingrid,
Wehrens Ron,
Stunnenberg Hendrik
Publication year - 2007
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601550
Subject(s) - biology , identification (biology) , computational biology , genetics , ecology
Our current knowledge of the general factor requirement in transcription by the three mammalian RNA polymerases is based on a small number of model promoters. Here, we present a comprehensive chromatin immunoprecipitation (ChIP)‐on‐chip analysis for 28 transcription factors on a large set of known and novel TATA‐binding protein (TBP)‐binding sites experimentally identified via ChIP cloning. A large fraction of identified TBP‐binding sites is located in introns or lacks a gene/mRNA annotation and is found to direct transcription. Integrated analysis of the ChIP‐on‐chip data and functional studies revealed that TAF12 hitherto regarded as RNA polymerase II (RNAP II)‐specific was found to be also involved in RNAP I transcription. Distinct profiles for general transcription factors and TAF‐containing complexes were uncovered for RNAP II promoters located in CpG and non‐CpG islands suggesting distinct transcription initiation pathways. Our study broadens the spectrum of general transcription factor function and uncovers a plethora of novel, functional TBP‐binding sites in the human genome.