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Regulating angiogenesis at the level of PtdIns‐4,5‐P 2
Author(s) -
Im Eunok,
Kazlauskas Andrius
Publication year - 2006
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/sj.emboj.7601100
Subject(s) - angiogenesis , biology , phosphatidylinositol , signal transduction , microbiology and biotechnology , pi3k/akt/mtor pathway , phospholipase c , kinase , biochemistry , genetics
Angiogenesis is a coordinated sequence of cellular responses that result in the outgrowth of new blood vessels. The angiogenic program is regulated by extracellular factors, whose input is integrated at least in part at the level of signal transduction pathways driven by phosphoinositide 3 kinase (PI3K) and phospholipase Cγ (PLCγ). Using an in vitro angiogenesis model, we discovered that PI3K was essential for tube formation, whereas PLCγ promoted regression. The underlying mechanism by which PLCγ antagonized tube formation appeared to be by competing with PI3K for their common substrate, phosphatidylinositol‐4,5‐bisphosphate. These studies are the first to identify signaling enzymes involved with vessel regression, and reveal that the angiogenic program can be coordinated by the availability of a membrane lipid.