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ASPECTS OF STIMULATION OF BONE MARROW COLONY GROWTH IN VITRO
Author(s) -
Bradley TR
Publication year - 1968
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1968.27
Subject(s) - trypsinization , bone marrow , in vitro , biology , stimulation , colony stimulating factor , andrology , microbiology and biotechnology , endocrinology , stem cell , immunology , medicine , haematopoiesis , trypsin , biochemistry , enzyme
Summary Cells from the kidneys of embryonic mice (17–19th day of gestation) were shown to be inactive with respect to the stimulation of colony growth of adult mouse bone marrow cells in vitro , whilst kidney cells from 2‐, 8‐ or 14‐day‐old mice were active in the induction of colony growth. Colony stimulating activity decreased piogiessively with cells from the kidneys of 1‐, 2‐ or 3‐month‐old mice. In vitro marrow cell colony stimulating activity was shown to be related to the number of kidney feeder cells used but was not confined to marrow cells of the same mouse strain. Similar colony stimulating activity was shown by trypsinized mouse embryo cells, and cells from several tissues were shown not to contribute to this activity. Contact between the feeder layers and bone marrow cells was not essential for colony development.