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METHODS FOR THE SEPARATION AND IDENTIFICATION OF THE IODINE‐CONTAINING AMINO ACIDS IN HUMAN PLASMA
Author(s) -
Wellby Maurice L
Publication year - 1962
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1962.45
Subject(s) - diiodotyrosine , chemistry , iodine , amino acid , chromatography , decomposition , thiouracil , extraction (chemistry) , chloride , organic chemistry , biochemistry , thyroid , medicine , hormone , triiodothyronine
SUMMARY A method of extracting and identifying the individual iodine‐containing amino acids in plasma is described. It makes use of an anion exchange resin (Dowex 1) for the extraction of the iodinated compounds, paper chromatography for their separation and the application of the ceric sulphate‐arsenious acid reaction for their detection on the chromatogram. The method makes possible the chemical detection of 0·5 μg./100 ml. of diiodotyrosine (DIT) and 0·5 μg./100 ml. of thyroxine (T4) in 6 ml. of plasma. No evidence of decomposition of T4 or T3, either radioactively labelled or not, was found during the procedure. The behaviour of related iodine‐containing amino acids and other compounds such as chloride and thiouracil derivatives were also investigated.