Open AccessCharacterization of hobo element copy number and integrity in Brasilian populations of Drosophila melanogaster
Author(s) -
RUIZ MARIANGELA TORREGLOSA,
CARARETO CLAUDIA MARCIA APARECIDA
Publication year - 2003
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1034/j.1601-5223.2003.01708.x
Subject(s) - biology , transposable element , xhoi , drosophila melanogaster , genetics , p element , restriction enzyme , southern blot , gene , genome , ecori
We have determined the copy number and the presence of full‐size hobo transposable elements in eight Brasilian strains of Drosophila melanogaster. G enomic DNA was digested with Ava II and Xho I restriction enzymes, respectively, and probed with a 963 bp sequence of the hobo element. Variable numbers of full‐sized and defective elements were detected in all strains. The range of the copy number was 22.13±4.52. Blots showed the presence of a 2.6 kb fragment, corresponding to the complete element, in all strains exception of one and the 1.0 kb sequence, correponding to the Th 1 and Th 2 repressor elements. There was neither association among copy numbers of hobo elements and latitude nor the mean annual temperatures in the original geographical region of each strain.