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Actin Assembly Plays a Variable, but not Obligatory Role in Receptor‐Mediated Endocytosis
Author(s) -
Fujimoto L. Miya,
Roth Robyn,
Heuser John E.,
Schmid Sandra L.
Publication year - 2000
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1034/j.1600-0854.2000.010208.x
Subject(s) - endocytosis , microbiology and biotechnology , biology , endocytic cycle , cytochalasin d , actin cytoskeleton , receptor mediated endocytosis , cytochalasin , mdia1 , actin , cytoskeleton , actin remodeling , cytochalasin b , bulk endocytosis , cell , biochemistry
Three cell‐permeant compounds, cytochalasin D, latrunculin A and jasplakinolide, which perturb intracellular actin dynamics by distinct mechanisms, were used to probe the role of filamentous actin and actin assembly in clathrin‐mediated endocytosis in mammalian cells. These compounds had variable effects on receptor‐mediated endocytosis of transferrin that depended on both the cell line and the experimental protocol employed. Endocytosis in A431 cells assayed in suspension was inhibited by latrunculin A and jasplakinolide, but resistant to cytochalasin D, whereas neither compound inhibited endocytosis in adherent A431 cells. In contrast, endocytosis in adherent CHO cells was more sensitive to disruption of the actin cytoskeleton than endocytosis in CHO cells grown or assayed in suspension. Endocytosis in other cell types, including nonadherent K562 human erythroleukemic cells or adherent Cos‐7 cells was unaffected by disruption of the actin cytoskeleton. While it remains possible that actin filaments can play an accessory role in receptor‐mediated endocytosis, these discordant results indicate that actin assembly does not play an obligatory role in endocytic coated vesicle formation in cultured mammalian cells.