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Comparison of apoptosis and apoptosis‐related gene products between extranodal oral B‐cell lymphoma and maxillofacial nodal B ‐ cell lymphoma
Author(s) -
Yin Hongfang,
Okada Norihiko,
Takagi Minoru
Publication year - 2001
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2001.300303.x
Subject(s) - lymphoma , pathology , tunel assay , medicine , apoptosis , diffuse large b cell lymphoma , b cell , immunohistochemistry , nodal , biology , immunology , antibody , biochemistry
Twenty‐seven cases of primary extranodal oral B‐cell lymphoma and 22 cases of primary maxillofacial nodal B‐cell lymphoma were investigated for the presence of apoptotic cells and the expression of apoptosis‐related gene products by terminal deoxynucleotidyl‐mediated dUTP nick end labeling (TUNEL) and immunohistochemistry. The majority of extranodal oral diffuse large B‐cell lymphomas (17/25, 68%) and maxillofacial nodal diffuse large B‐cell lymphomas (14/16, 88%) contained no or less than 10% apoptotic cells. Whereas the majority of extranodal oral diffuse large B‐cell lymphomas (18/25, 72%) and maxillofacial nodal diffuse large B‐cell lymphomas (13/16, 81%) contained more than 10% of Ki‐67‐positive cells. Bcl‐2‐, Bax‐, p53‐ and Ki‐67‐positive rates were higher in maxillofacial nodal diffuse large B‐cell lymphomas than in extranodal oral diffuse large B‐cell lymphomas, but only Bax (χ 2 test, 0.01< P <0.025) and p53 (χ 2 test, 0.005< P <0.01) had significant differences. Extranodal oral diffuse large B‐cell lymphomas had a higher frequency of TUNEL expression than maxillofacial nodal diffuse large B‐cell lymphomas. In maxillofacial nodal diffuse large B‐cell lymphomas, stage III and stage IV tumors had a significantly higher frequency of Bcl‐2 expression than stage I and stage II tumors (Fisher’s exact test, P <0.01). These findings indicated that in the majority of both extranodal oral and maxillofacial nodal diffuse large B‐cell lymphomas, apoptosis was inhibited – whereas proliferative activity was accelerated. Impairment of apoptosis and apoptotic related gene products may have a more important relation to maxillofacial nodal diffuse large B‐cell lymphoma than extranodal oral diffuse large B‐cell lymphoma.