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Lack of association between Streptococcus oralis and recurrent aphthous stomatitis
Author(s) -
Riggio Marcello P.,
Len Alan,
Ghodratnama Fatemeh,
Wray David
Publication year - 2000
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2000.290105.x
Subject(s) - streptococcus oralis , streptococcus mitis , polymerase chain reaction , biology , hindiii , restriction enzyme , streptococcus , microbiology and biotechnology , genetics , dna , gene , bacteria
In the present study, the potential involvement of Streptococcus oralis in the aetiology of recurrent aphthous stomatitis (RAS) was investigated using the polymerase chain reaction (PCR). Biopsies from 28 RAS patients were analysed, in addition to 20 oral lichen planus (OLP) and 13 normal biopsies that were used as controls. PCR was carried out using a primer pair that targets the D ‐alanine: D ‐alanine ligase gene and detects DNA from both S. oralis and the closely related species Streptococcus mitis . Discrimination between these two species was achieved by digestion of PCR products with the restriction endonucleases Hae III and Hin dIII, which both give distinct restriction profiles for each species. S. oralis DNA was detected in 8 of 28 (29%) RAS samples, 10 of 20 (50%) OLP samples and 6 of 13 (46%) normal samples. These results suggest that S. oralis is not of primary aetiological significance in RAS.