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Expression of HGF/SF and Met protein is associated with genetic alterations of VHL gene in primary renal cell carcinomas
Author(s) -
Oh Ro Ra,
Park Jik Young,
Lee Jong Heun,
Shin Min Sun,
Kim Hong Sug,
Lee Sang Kyu,
Kim Young Sil,
Lee Sug Hyung,
Lee Sie Nae,
Yang Young Mok,
Yoo Nam Jin,
Lee Jung Young,
Park Won Sang
Publication year - 2002
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1034/j.1600-0463.2002.100305.x
Subject(s) - missense mutation , biology , gene , cancer research , cell , hepatocyte growth factor , mutation , microbiology and biotechnology , genetics , receptor
We analyzed the genetic alterations of VHL , HGF/SF , and Met genes and the expression pattern of HGF/SF and Met protein in 26 renal cell carcinomas (RCCs). We found five mutations of the VHL gene and frequent LOH (50%) only in non‐papillary clear cell RCC. We found six cases in which the CpG island of VHL was methylated. In addition, one missense mutation of the HGF/SF gene was detected in clear cell RCC. HGF/SF and Met protein were expressed in 84.6% and 80.7% of RCCs, respectively. All of the cases with the genetic alterations of VHL or HGF/SF demonstrated strong expression of HGF/SF and Met protein in RCC cells. Statistically, genetic alterations of VHL and HGF/SF were significantly correlated with HGF/SF and Met expression (Fisher's exact test, p=0.022 and p=0.0070). Thus, these results strongly suggest that the expression of HGF/SF and Met protein is closely associated with the genetic alterations of VHL and HGF/SF in primary RCCs.